![]() While DNA sequencing by CE is used to determine the specific base sequence of a particular fragment or gene fragment, fragment analysis can provide sizing, relative quantitation, and genotyping analysis for fluorescently labeled DNA fragments produced by PCR using primers designed for a specific DNA target. Fragment analysis comprises a series of techniques in which DNA fragments are fluorescently labeled, separated by capillary electrophoresis (CE), and sized by comparison to an internal standard. RFLP is widely used in genetic and genomic research, such as genome mapping and gene identification. Introduction Restriction Fragment Length Polymorphism (RFLP) is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA samples in question with specific restriction endonucleases. Fragment analysis is faster and less tedious than RFLP. We developed an alternative nested-PCR-restriction fragment length polymorphism (RFLP) protocol for the detection of Cyclospora cayetanensis in environmental samples that obviates the need for microscopic examination. Abstract RFLP (Restriction Fragment Length Polymorphism) is a commonly used technique that can be used for genotyping for nearly all organisms, including plants, animals, and humans. This versatile method employs PCR amplification of genomic DNA extracted from fish samples, followed by restriction fragment length polymorphism (RFLP).
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